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GrainGenes Marker Report: CFD79

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Locus	cfd79
Type	Microsatellite
Map	Durum Wheat, NSGC Lr 3B 18.6 Physical-3B 18.3 Wheat, Yr genes and QTL 3A 13 Wheat, Yr genes and QTL 3B 20.8 Wheat_Consensus_2014_Ug99_Rust_3B 26.11
	[ Show Nearby Loci ]
Map Data	3B Physical Durum Wheat, NSGC Lr Wheat, Yr genes and QTL Wheat_Consensus_2014_Ug99_Rust
Probe	CFD79
Possible Orthologs	Xcfd79-3A [ Show all 15 ]
Probe	CFD79
Locus	Xcfd79-3B [ Show all 16 ]
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
Remarks	Quality Score = D Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)26 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' TCTGGTTCTTGGGAGGAAGA 3' Reverse 5' CATCCAACAATTTGCCCAT 3'
SSR size	284
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	3D/3B
Source Species	Aegilops tauschii