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GrainGenes Marker Report: CFD52

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Probe	CFD52
Locus	Xcfd52-5D
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: ? Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' AGACGGTGTTGGTTCCTGAG 3' Reverse 5' TAGTGGCCGGTGGAATTAAA 3'
SSR size	275
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	5D
Source Species	Aegilops tauschii