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GrainGenes Marker Report: CFD127

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Probe	CFD127
Locus	Xcfd127-3D Xcfd127
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
Remarks	Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GCC)6 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' TAAACACCAGGGAGGTCCAC 3' Reverse 5' ACCTACGATCGACGAAATGG 3'
SSR size	150
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	3D
Source Species	Aegilops tauschii