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GrainGenes Marker Report: CFA2149

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Probe	CFA2149
Locus	Xcfa2149-4B Xcfa2149-5A Yr48 Xcfa2149
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157. Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167. Hegarty J et al. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Stripe Rust Resistance. Yr48 MAS Wheat. Marker Assisted Selection in Wheat.
Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (TG)20 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' CTTGGAGCTCGGGTAGTAGC 3' Reverse 5' AAGGCAGCTCAATCGGAGTA 3'
SSR size	231
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	4B
Source Gene	Yr48 (Triticum)
Source Species	Triticum urartu