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GrainGenes Marker Report: Bmag013

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Locus	Bmag013
Type	Microsatellite
QTL with Locus Marker	QPcx.TaER-3H.3 QYld.TaER-3H.3
Probe	Bmag13
Possible Orthologs	Bmag0013 Bmag13
Probe for Locus	Bmag13
Locus	Bmag0013 Bmag13 Bmag013
Synonym	Other name Bmag0013
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (CT)21 Quality = 4: Ladders of bands that are at equal intensity, but may contain scoreable weak products Diversity Index (DI) = 0.72
Type	PCR SSR
PCR primers	5' AAGGGGAATCAAAATGGGAG 3' 5' TCGAATAGGTCTCCGAAGAAA 3'
SSR size	155
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare