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GrainGenes Marker Report: Bmac187

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Locus	Bmac187
Type	Microsatellite
Synonym	Synonym Bmac0187
Chromosome	7H
Map	Hordeum-Consensus2006-DArT-7H 75.2
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Map Data	Barley, Consensus 2006, DArT
Probe	Bmac187
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Remark	Number of populations contributing to the placement of Bmac187 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	Bmac0187 Bmac187-7H
Probe	Bmac187
Locus	Bmac0187 Bmac187-7H Bmac187
Synonym	Other name Bmac0187
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)13(AT)9 Quality = 2: Single product, relatively strong stutter bands
Type	PCR SSR
PCR primers	5' GCTCTCTCTCAGAAAAATGAA 3' 5' GAATTATTCTAGGGCTGTGAA 3' GCTCTCTCTCAGAAAAATGAA GAATTATTCTAGGGCTGTGAA
SSR size	177
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare