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GrainGenes Marker Report: Bmac047B

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Locus	Bmac047B
Type	Microsatellite
Chromosome	7H
Map	Hordeum-OWB-7H 65.9
	[ Show Nearby Loci ]
Map Data	Barley, OWB
Probe	Bmac47
Possible Orthologs	Bmac0047 [ Show all 8 ]
Probe for Locus	Bmac47
Locus	Bmac0047b [ Show all 8 ]
Synonym	Other name Bmac0047
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)16 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type	PCR SSR
PCR primers	5' AACACACGTACACAAATACACA 3' 5' ACGTCCATCACTTTGACC 3'
SSR size	190
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare