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GrainGenes Marker Report: BMS64

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Locus	BMS64
Type	Microsatellite
Chromosome	7H
Map	Hordeum-Lx41-7H 143.3 Hordeum-Pillen-7H 98
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QTL with Locus Marker	QHea.pil-7H.1 QHei.pil-7H.1 QWa.pil-7H.1
Map Data	Barley, AxHs/QTL Barley, Lx41
Probe	Bmac64 BMS64
Probe for Locus	Bmac64
Locus	Bmac0064 BMS64 Bmac64
Synonym	Other name Bmac0064 BMS64
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)21 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type	PCR SSR
PCR primers	5' CTGCAGGTTTCAGGAAGG 3' 5' AGATGCCCGCAAAGAGTT 3'
SSR size	155
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare