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GrainGenes Marker Report: Alt2

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Locus	Alt2
Type	Trait
Chromosome	4D
Chromosome Arm	4DL
Map	Wheat-Composite2004-4D 60
	[ Show Nearby Loci ]
Species	Triticum aestivum
Probe	WMC331
Associated Gene	Alt2 (Triticum)
Gene Class	Aluminium tolerance Aluminum tolerance
Reference	Raman H et al. (2005) Molecular characterization and mapping of ALMT1, the aluminium-tolerance gene of bread wheat (Triticum aestivum L.). Genome 48:781-791.
Remark	Trait marker Xwmc48b, Xwmc331 Nearby marker on Composite map: Xwmc331 Position on Composite map estimated by GrainGenes.
Probe for Locus	WMC331
Locus	Xwmc331-4D Xwmc331 Alt2 wmc331
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 17f11 Donor: Khairallah, Mexico Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 134 bases located on 4D Trimmed to insert at 5' end and to insert at 3' end. (CA)13 56 to 81, (CT)10 36 to 55. Software struggled to get these primers.
Type	PCR SSR
PCR primers	Forward ccTgTTgcATAcTTgAccTTTTT at 2 length 23 bases, Tm 61 Reverse ggAgTTcAATcTTTcATcAccAT at 107 length 23 bases, Tm 61
SSR size	128 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide.
Sequence	WMC331
Source Species	Triticum aestivum