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GrainGenes Marker Report: AM7

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Locus
am7
Type
Microsatellite
Synonym
Other nameAM7
SynonymAM0007
AM7
Map Data
Oat, KxO, 2003
Species
Avena sativa
Probe
AM7
Remark
Marker placement value = 0.00 cM on the Oat-KxO/2003 maps
Marker is unlinked on the Oat-KxO/2003 maps
Possible Orthologs
AM7
am7a
am7b
AM0007



Probe
AM7
Locus
am7
AM7
am7a
am7b
AM0007
Reference
ReferenceLi CD et al. (2000) The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars Theoretical and Applied Genetics 101:1259-1268.
Remarks
Insert contains repeat (AG)21
Known repeat = 5'-Alu with 75 % identity over 62 base pairs
Polymorphic in Kanota/Ogle and Marion/Terra.
Among 20 oat cultivars, 1 allele was found.
Type
PCR
SSR
PCR primers
5'GTG AGC GCC GAA TAC ATA
5'TTG GCT AGC TGC TTG AAA CT
SSR size
156
Amplification Conditions
Average melting temperature (degrees C) = 48
Source Species
Avena sativa
Source Germplasm
Ogle (oat)