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GrainGenes Marker Report: AM5

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Locus	AM5
Type	Microsatellite
Map	Oat-AxM-DH2008-7 48
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Map Data	Oat, AxM Doubled Haploid, 2008
Species	Avena sativa
Probe	AM5
Reference	Tanhuanpaa P et al. (2008) The first doubled haploid linkage map for cultivated oat. Genome 51:560-569.
Probe	AM5
Locus	AM5
Reference	Fu YB et al. (2007) Microsatellite variation in Avena sterilis oat germplasm Theoretical and Applied Genetics 114:1029-1038. Li CD et al. (2000) The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars Theoretical and Applied Genetics 101:1259-1268.
Remarks	Insert contains repeat (AG)27 Known repeat = 5'-Alu with 72 % identity over 47 base pairs In 369 Avena sterilis accessions there were 4 alleles of size 134-142 bp, PIC = 0.09.
Type	Genomic PCR SSR Microsatellite
PCR primers	5'TTG TCA GCG AAA TAA GCA GAG A 5'GAA TTC GTG ACC AGC AAC AG
SSR size	172
Amplification Conditions	Average melting temperature (degrees C) = 46
Source Species	Avena sativa
Source Germplasm	Ogle (oat)