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GrainGenes Marker Report: AM4

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Locus	AM4
Type	Microsatellite
Map	Oat-AxM-DH2008-2 22
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Map Data	Oat, AxM Doubled Haploid, 2008
Species	Avena sativa
Probe	AM4
Reference	Tanhuanpaa P et al. (2008) The first doubled haploid linkage map for cultivated oat. Genome 51:560-569.
Probe	AM4
Locus	AM4
Reference	Fu YB et al. (2007) Microsatellite variation in Avena sterilis oat germplasm Theoretical and Applied Genetics 114:1029-1038. Li CD et al. (2000) The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars Theoretical and Applied Genetics 101:1259-1268.
Remarks	Insert contains repeat (AG)34 Known repeat = 3'-Alu with 70 % identity over 51 base pairs Polymorphic in Kanota/Ogle and Marion/Terra. Among 20 oat cultivars, 5 alleles were found, polymorphic information content (PIC) = 0.68. In 369 Avena sterilis accessions there were 21 alleles of size 114-220 bp, PIC = 0.89.
Type	Genomic PCR SSR Microsatellite
PCR primers	5'GGT AAG GTT TCG AAG AGC AAA G 5'GGG CTA TAT CCA TCC CTC AC
SSR size	166
Amplification Conditions	Average melting temperature (degrees C) = 48
Source Species	Avena sativa
Source Germplasm	Ogle (oat)