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GrainGenes Marker Report: AM31

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Locus
AM31
Type
Microsatellite
Map
Oat-AxM-DH2008-1667
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Map Data
Oat, AxM Doubled Haploid, 2008
Species
Avena sativa
Probe
AM31
Reference
ReferenceTanhuanpaa P et al. (2008) The first doubled haploid linkage map for cultivated oat. Genome 51:560-569.



Probe
AM31
Locus
AM31
Reference
ReferenceLi CD et al. (2000) The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars Theoretical and Applied Genetics 101:1259-1268.
Remarks
Insert contains repeat (GAA)23
Among 20 oat cultivars, 3 alleles were found, polymorphic information content (PIC) = 0.51.
Type
PCR
SSR
Microsatellite
PCR primers
5'GCA AAG GCC ATA TGG TGA GAA
5'CAT AGG TTT GCC ATT CGT GGT
SSR size
186
Amplification Conditions
Average melting temperature (degrees C) = 47
Source Species
Avena sativa
Source Germplasm
Ogle (oat)