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GrainGenes Marker Report: AM3

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Locus	AM3
Type	Microsatellite
Synonym	Synonym AM0003
Map	Oat-2009-KxO_36 20 [ Show all 10 ]
	[ Show Nearby Loci ]
Map Data	Oat, AxM Doubled Haploid, 2008 [ Show all 7 ]
Species	Avena sativa
Probe	AM3
Reference	Tanhuanpaa P et al. (2008) The first doubled haploid linkage map for cultivated oat. Genome 51:560-569.
Remark	Marker placement value = 7.58 cM on map Oat-KxO/2003-36 Marker is not a framework marker on map Oat-KxO/2003-36
Probe	AM3
Locus	AM3 AM0003
Reference	Fu YB et al. (2007) Microsatellite variation in Avena sterilis oat germplasm Theoretical and Applied Genetics 114:1029-1038. Li CD et al. (2000) The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars Theoretical and Applied Genetics 101:1259-1268.
Remarks	Insert contains repeat (AG)35 Polymorphic in Kanota/Ogle. Among 20 oat cultivars, 5 alleles were found, polymorphic information content (PIC) = 0.73. In 369 Avena sterilis accessions there were 24 alleles of size 144-378 bp, PIC = 0.93.
Type	Genomic PCR SSR Microsatellite
PCR primers	5'CTG GTC ATC CTC GCC GTT CA 5'CAT TTA GCC AGG TTG CCA GGT C
SSR size	280
Amplification Conditions	Average melting temperature (degrees C) = 51
Source Species	Avena sativa
Source Germplasm	Ogle (oat)