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GrainGenes Marker Report: AM1b

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Locus	AM1b
Type	Microsatellite
Synonym	Synonym am1b
Map	Oat-AxM-DH2008-24 16
	[ Show Nearby Loci ]
Map Data	Oat, AxM Doubled Haploid, 2008
Species	Avena sativa
Probe	AM1
Reference	Tanhuanpaa P et al. (2008) The first doubled haploid linkage map for cultivated oat. Genome 51:560-569.
Possible Orthologs	am1 [ Show all 7 ]
Probe for Locus	AM1
Locus	AM1a [ Show all 8 ]
Reference	Fu YB et al. (2007) Microsatellite variation in Avena sterilis oat germplasm Theoretical and Applied Genetics 114:1029-1038. Li CD et al. (2000) The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars Theoretical and Applied Genetics 101:1259-1268.
General Remarks	Insert contains repeat (AG)21(CAGAG)6 Polymorphic in Kanota/Ogle and Marion/Terra. Among 20 oat cultivars, alleles at two loci were found. There were 8 alleles at locus AM1a, polymorphic information content (PIC) = 0.85, and 2 alleles at AM1b, PIC = 0.18. In 369 Avena sterilis accessions there were 11 alleles of size 150-240 bp, PIC = 0.41.
Type	Genomic PCR SSR Microsatellite
PCR primers	5'GGA TCC TCC ACG CTG TTG A 5'CTC ATC CGT ATG GGC TTT A
SSR size	204
Amplification Conditions	Average melting temperature (degrees C) = 46
Source Species	Avena sativa
Source Germplasm	Ogle (oat)