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GrainGenes Marker Report: ABG399B

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Locus	ABG399B
Type	RFLP
Map	Barley-Consensus2-7H 3.1 Hordeum-Bins-7H 1 Hordeum-KxM-7H 0
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Map Data	Barley consensus 2 Barley, BinMap 2005 Barley, HxT Barley, KxM
Probe	ABG399
Data Source	Kleinhofs, Andris
Remark	This marker is a Binlocus in the Hordeum-Bins maps, i.e. a marker mapped in other crosses and located to the Steptoe x Morex bin
Possible Orthologs	ABG399 ABG399A Xabg399
Probe for Locus	ABG399
Locus	ABG399B Xabg399-3 ABG399 ABG399A
General Remarks	ABG399 has two inserts, 0.7 and 0.5 kb. The 0.5 kb fragment gives a Very clean blot with few bands and should be used for mapping. Harrington has a very strong band with this fragment that is absent in the other 3 parents. The 0.7 fragment gives multiple bands and messy blot. Will reclone the 0.5 kb band in a separate plasmid.
Type	Genomic
Linkage Group	3
Source Species	Hordeum vulgare
Source Germplasm	Shin Ebisu 16
Insert Size	0.5