TriFLDB is a database that provides full-length coding sequences currently available for wheat and barley and their annotations. The current version of TriFLDB houses 7,341 putative full-length coding sequences and their annotations in the case of barley and 8,530 in the case of wheat; this includes data derived from 5,006 barley full-length cDNA sequences retrieved from BarleyDB of Research Institute for Bioresources, Okayama University and from 6,162 wheat full-length cDNA sequences (3.2 MB) (6,137 filtered putative FLCDS set) that were newly sequenced in RIKEN and Kihara Institute for Biological Research, Yokohama City University supported by National Bio Resource Project in Japan.