IWGSC Reference Sequence v1.0 Annotation NOW available at URGI

The International Wheat Genome Sequencing Consortium is pleased to announce that the first version of the reference sequence of the bread wheat variety Chinese Spring (IWGSC RefSeq v1.0) is now available with annotation of genes, non-coding RNAs and transposable elements at the IWGSC sequence repository hosted by URGI-INRA .

The pre-publication data are being made available under the IWGSC general data access agreement which is consistent with the Toronto Agreement and that grants the IWGSC the right to publish the first global analyses of the data. This includes descriptions of whole chromosome or genome-level analyses of genes, gene families, repetitive elements, and comparisons with other organisms.

The IWGSC RefSeq v1.0 annotation includes gene models generated by integrating predictions made by INRA-GDEC using Triannot and PGSB using their customised pipeline (previously MIPS pipeline). The integration was undertaken by the Earlham institute (EI), who have also added UTRs to the gene models where supporting data are available. Gene models have been assigned to high confidence (HC) or low confidence (LC) classes based on completeness, similarity to genes represented in protein and DNA databases and repeat content. The automated assignment of functional annotation to genes has been generated by PGSB based on AHRD parameters. In addition, annotated transposable elements (TEs) and non-coding RNAs are available. More information about the annotation data is provided in README files from the IWGSC repository hosted on the URGI Sequence Repository .

How to access the data

Access does require registration and agreeing to respect the right of the IWGSC to publish first. For specific access terms, see the IWGSC General Data Access agreement .

  • Individuals who have not signed the IWGSC Data Access Agreement should FIRST register on the IWGSC website and sign the Agreement; URGI login details will be provided subsequently for access to the data.
  • Individuals who have already signed the IWGSC Data Access Agreement can go directly to the URGI website to access the data using their URGI login details.

Jan. 2017 notice:
The International Wheat Genome Sequencing Consortium is pleased to announce that the first version of the reference sequence of the bread wheat variety Chinese Spring (IWGSC RefSeq v1.0) is now available at the IWGSC sequence repository hosted by URGI-INRA (https://wheat-urgi.versailles.inra.fr/Seq-Repository/Assemblies)

The IWGSC RefSeq v1.0 is an integration of the IWGSC WGA v0.4 – made available in June 2016 – with IWGSC chromosome-based and other resources, including but not limited to:

  • Physical maps for all chromosomes;
  • Sequenced BACs for 8 chromosomes (1A, 1B, 3B, 3D, 6B, 7A, 7B, 7D) and partial MTP BAC sequences for 2 chromosome arms (4AL, 5BS);
  • MTP BAC WGPTM sequence tags for all chromosomes, except 3B;
  • BioNano optical maps (7A, 7B, 7DS);
  • Alignment to RH maps (D chromosomes); and
  • GBS map of the SynOp RIL population CsxRn genetic map (INRA).

With the addition of the resources that have been developed by IWGSC members over the past few years, the quality of the assembly increased substantially. When compared with IWGSC WGA v0.4, the chromosomal scaffold/ superscaffold N50 increased from 7.0 Mb to 22.8 Mb.

The data are available for BLAST searches and can be downloaded from the IWGSC repository hosted on the URGI Sequence Repository .
How to access the data

Earlier Notice:
The IWGSC is pleased to announce that the Genome Assembly (IWGSC WGA) of bread wheat, *T. aestivum* cv Chinese Spring (ERGE 2135), based on Illumina short sequence reads assembled with NRGene's DeNovoMAGICTM software is now available at the IWGSC sequence repository hosted by URGI-INRA.

A BLAST server has been set up to facilitate rapid access to single or small numbers of queries. The data can also be downloaded from the URGI IWGSC repository.

The data are being made available before publication in accordance with the Toronto Agreement under which the IWGSC reserves the right to publish the first global analyses of the data. This includes descriptions of whole chromosome or genome-level analyses of genes, gene families, repetitive elements, and comparisons with other organisms.

Access does require registration and agreeing to the IWGSC General Data Access terms.

*Useful links:*

  • Web announcement details on the data available, how to access them and links to URGI BLAST
    and download
  • Press release: Wheat Sequencing Consortium Releases Key Resource to the Scientific Community

Best regards

The IWGSC team