GrainGenes Trait Study Report: Diaphorase activity, Souza88
Trait Study
Diaphorase activity, Souza88
Trait
Diaphorase activity
Reference
Souza E (1988) Measures and applications of genetic relationships in oats.
Protocol
Diaphorase and esterase stains were assayed on horizontal slices of as single gel. Diaphorase activity was assayed by incubating gel slices at 37 deg C in 50 ml of a solution of 2.0 x 10^-4^ M 2,6 dichlorophenol indophenol, 4.8 x 10^-4^ M 3-(4,5-Dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT), 2.0 x 10^-4^ M NADH, and 0.1 M Tris (Weeden, 1984). Diaphorase enzyme activity was found in six well defined zones at Rf 0.82, 0.79, 0.76, 0.29, 0.25, 0.21. Additionally, there was a zone of diffuse activity from Rf 0.60 to 0.45. Five of the six zones were invariant in the cultures surveyed. However, the isozyme at Rf 0.76 was not present in 17 of the 70 cultivars. The null zone was found in 59% of fall planted cultivars while only 3 spring cultivars were found to be homogeneous for the null zone and a single Spring cultivar, multiline Multiline E77 (oat), was heterogeneous for the null phenotype. Dal was the only recent spring cultivar with the null Rf 0.76 isozyme. The other two were Green Mountain and Joanette, a historical cultivar and a parental source of stem rust resistance, respectively. Crosses between Ogle (Rf 0.76 isozyme present) and two parents with the null allele were examined for segregation of a null allele at the Rf 0.76 zone. In the Ogle/White Lustre and the Ogle/Windsor crosses, the segregation for the null allele fit the 3:1 segregation of a dominant gene at a single locus, designated for this paper, Dia-1 with alternate alleles Dia1(present) and dia1 (absent).
Values
Dia1 = active allele dia1 = null allele
Germplasm Description
[ Hide all but 1 of 70 ] Advance (oat)
Dia1 active allele Appaloosa (oat)
Dia1 active allele Astro (oat)
Dia1 active allele Banner (oat)
Dia1 active allele Bates (oat)
Dia1 active allele Bond (oat)
Dia1 active allele Brooks (oat)
Dia1 active allele Calibre (oat)
Dia1 active allele Cascade PI496252
Dia1 active allele Ceal (oat)
Dia1 active allele Centennial (oat)
Dia1 active allele Chilocco (oat)
dia1 null allele Cimarron (oat)
dia1 null allele Clintford (oat)
Dia1 active allele Clintland 64 (oat)
Dia1 active allele Coker 227 (oat)
dia1 null allele Coker 716 (oat)
dia1 null allele Corbit (oat)
Dia1 active allele Cornellian (oat)
Dia1 active allele Dal (oat)
dia1 null allele Donald (oat)
Dia1 active allele Elan (oat)
dia1 null allele Fidler (oat)
Dia1 active allele Florida 501 (oat)
dia1 null allele Fulghum (oat)
Dia1 active allele Garry CIav6662
Dia1 active allele Gopher (oat)
Dia1 active allele Green Mountain (oat)
dia1 null allele Hairy Culberson (oat)
dia1 null allele Hajira (oat)
Dia1 active allele Hercules (oat)
Dia1 active allele Hudson (oat)
Dia1 active allele Jaycee (oat)
Dia1 active allele Joanette (oat)
dia1 null allele Kelsey (oat)
Dia1 active allele Kherson (oat)
Dia1 active allele Lamar (oat)
Dia1 active allele Lancer (oat)
Dia1 active allele Lang (oat)
Dia1 active allele Mackinaw (oat)
Dia1 active allele Marathon (oat)
Dia1 active allele Marion (oat)
Dia1 active allele Mesa (oat)
Dia1 active allele Moore (oat)
Dia1 active allele Multiline E77 (oat)
dia1 null allele Multiline M73 (oat)
Dia1 active allele Nodaway 70 (oat)
Dia1 active allele Norline CIav6903
dia1 null allele Ogle (oat)
Dia1 active allele Orbit (oat)
Dia1 active allele Otana CIav9252
Dia1 active allele Otee (oat)
Dia1 active allele PA7507-015
Dia1 active allele Pierce (oat)
Dia1 active allele Porter (oat)
Dia1 active allele Red Rustproof (oat)
Dia1 active allele Salem (oat)
Dia1 active allele SD 790400 (oat)
Dia1 active allele Shaw (oat)
Dia1 active allele Sierra (oat)
Dia1 active allele Spear (oat)
Dia1 active allele TAM O-301 (oat)
Dia1 active allele TAM O-312 (oat)
Dia1 active allele Trispernia (oat)
Dia1 active allele Walken (oat)
dia1 null allele White Tartar (oat)
Dia1 active allele Windsor (oat)
dia1 null allele Winter Turf (oat)
dia1 null allele Wintok (oat)
dia1 null allele Woodstock (oat)
Dia1 active allele
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Trait Study Report: Diaphorase activity, Souza88
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||