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GrainGenes Reference Report: PHY-91-232

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Reference	PHY-91-232
Title	Infection of winter wheat by a beta-glucuronidase-transformed isolate of Cephalosporium gramineum
Journal	Phytopathology
Year	2001
Volume	91
Pages	232-239
Author	Douhan GW Murray TD
Abstract	Summary: Field-grown winter wheat was inoculated with a beta-glucuronidase-transformed isolate of Cephalosporium gramineum in two field seasons to elucidate the mode of infection in resistant and susceptible cultivars. Colonization of viable root epidermis and cortical cells occurred as soon as 15 days postinoculation and the pathogen was found in the vascular tissues by 20 days postinoculation, well before freezing soil temperatures occurred. Penetration occurred directly through the root epidermis and through wounds adjacent to emerging secondary roots. The pathogen also penetrated through root cap cells and colonized meristematic tissues near root tips to gain access to the vascular system. Lower stem base colonization was observed where the pathogen penetrated directly through the epidermis, wounds, or senescent tissues. Appressorium-like structures, which appeared to aid penetration of cell walls, were often found within cells of both roots and stems after initial colonization. The mechanisms of resistance were not apparent, but less colonization occurred in resistant than in susceptible cultivars
Keyword	[ Hide all but 1 of 48 ] appressoria beta glucuronidase cell wall cephalosporium colonization cortex developmental stages disease resistance enzyme activity epidermis field freezing gene expression gene transfer genetic transformation genetic variation infection isolate mechanism pathogen pathogenicity pathotypes penetration plant anatomy plant pathogenic fungi resistance resistant root root tips seasonal variation soil soil temperature stem structure susceptibility symptoms temperature tissue transgenic plant triticum aestivum ultrastructure vascular system vascular tissues walls wild strains winter winter wheat wound

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