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GrainGenes Reference Report: PHY-84-684

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Reference	PHY-84-684
Title	Fungal zoospore-mediated delivery of a foreign gene to wheat roots
Journal	Phytopathology
Year	1994
Volume	84
Pages	684-687
Author	Zhang L Mitra A French R Langenberg W
Abstract	The primitive fungus Olpidium brassicae is an obligate plant parasite that acts as a natural virus vector transmitting tobacco necrosis virus and certain other viruses to roots of many monocotyledonous and dicotyledonous plants. Plasmid pAM981, carrying the chloramphenicol acetyltransferase (CAT) gene, was packaged by dissociated capsid protein of tobacco necrosis virus. The resulting nucleoprotein complexes were acquired by 0. brassicae zoospores and transmitted to wheat roots. Transient expression of CAT in wheat roots was detected, indicating that transformation can be achieved if plants can be regenerated from root tissue.
Keyword	[ Hide all but 1 of 11 ] chloramphenicol-acetyltransferase gene expression gene transfer genetic engineering genetic transformation genetic vectors olpidium-brassicae root tobacco-necrosis-necrovirus triticum aestivum zoospores

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