GrainGenes Reference Report: PHT-69-728
Reference
PHT-69-728
Title
Fluorescence analysis of oat phyA deletion mutants expressed in tobacco suggests that the N-terminal domain determines the photochemical and spectroscopic distinctions between phyA' and phyA''
Journal
Photochemistry and Photobiology
Year
1999
Volume
69
Pages
728-732
Author
Sineshchekov VA Clough RC Jordan-Beebe ET Vierstra RD
Abstract
Summary: In vivo low-temperature (85 K) fluorescence spectroscopy has defined two phytochrome A (phyA) subpopulations, designated phyA' and phyA'', in etiolated seedings (V. A. Sineshchekov, J. Photochem. Photobiol. 28, 53-55, 1995). Phytochrome A' is the more abundant but light-labile species characterized by longer wavelength emission/absorption maxima (687/673 nm) and by a higher extent of the photoconversion of its red-absorbing form (Pr) into photoproduct (lumi-R) at 85 K (gamma 1 approximately equal to 0.5). Phytochrome A'' is the minor but relatively light-stable species, characterized by shorter wavelength maxima (682/668 nm) and by a lower gamma 1 (<0.05). To help define domains within phyA responsible for these differences, the low-temperature spectral properties of transgenic tobacco expressing full-length (FL) oat phyA and C- and N-terminally truncated versions (CD [delta 786-1129] and NA [delta 7-69], respectively) were compared. Oat phytochrome expression was more pronounced than that of tobacco in the basal section of etiolated seedlings following 2 h irradiation with white light. Seedlings expressing FL and CD phyA had spectral properties for phyA' and phyA'' that were indistinguishable from that of wild-type tobacco. Conversely, expression of NA phyA generated an abundant phy species that behaved like phyA''. From this we conclude that the N-terminal domain of phyA is involved in determining the photochemical and spectroscopic distinctions between the native phyA' and phyA'' species
Keyword
[ Hide all but 1 of 7 ] avena sativa fluorescence light nicotiana tabacum phytochrome seedlings transgenic plant
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GrainGenes Reference Report: PHT-69-728
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||