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GrainGenes Reference Report: PEU-04-223

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Reference	PEU-04-223
Title	A retrotransposon sequence is related to DNA instability in barley microspore culture
Contained in	(2004) Genetic variation for plant breeding. Proceedings of the 17th EUCARPIA General Congress, Tulln, Austria, 8-11 September 2004.
Year	2004
Pages	223-225
Author	Munoz M [ Show all 8 ]
Abstract	To assay gametoclonal variation in barley, a set of doubled haploid (DH) plants (cv. 'Igri') regenerated from isolated microspore culture by direct embryogenesis, was especially developed. AFLP analysis was performed with eleven enzyme-primer combinations, that represented the study of 719 band polymorphisms. Only one change in the banding pattern was observed, indicating a high degree of genetic stability. An extra band was present in DH lines regenerated from microspores, treated or not with sodium azide, representing a 'hot spot' of instability. The sequence of this band was related to barley 'sukkula' retrotransposon element. Our preliminary results suggest the activation of retrotransposon by barley isolated microspore culture
Keyword	[ Hide all but 1 of 14 ] barley dna embryogenesis genetic polymorphism genetic stability genetic variation in vitro culture in vitro regeneration nucleotide sequence plant breeding retrotransposons sodium stability tissue culture

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