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GrainGenes Reference Report: BBA-1519-199

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Reference
BBA-1519-199
Title
Wheat mitochondria ccmB encodes the membrane domain of a putative ABC transporter involved in cytochrome c biogenesis
Journal
Biochimica et Biophysica Acta
Year
2001
Volume
1519
Pages
199-208
Author
Faivre-Nitschke SE
Nazoa P
Gualberto JM
Grienenberger JM
Bonnard G
Abstract
Summary: Assembly of cytochromes c is mediated by different proteins depending on the organism and organelle considered. In land plants, mitochondria follow a pathway distinct from that of yeast and animal mitochondria, more similar to that described for alpha- and gamma-proteobacteria. Indeed, in plant mitochondria, four genes were identified based on the similarities of their products with bacterial proteins involved in c-type cytochrome maturation. We report the characterisation of one of these mitochondrial genes in Triticum aestivum, TaccmB, which is proposed to encode a subunit of an ABC transporter. The transcript extremities were mapped and cDNA sequencing revealed 42 C to U editing positions in the 618 nucleotide long coding region. This high editing rate affects the identity of 32 amino acids out of 206. Antibodies directed against wheat CcmB recognise a 28 kDa protein in an enriched inner mitochondrial membrane protein fraction, a location which is in agreement with the high hydrophobicity of the protein and its function as a putative transmembrane domain of an ABC transporter involved in cytochrome c and c1 biogenesis in plant mitochondria
Keyword
[ Hide all but 1 of 48 ]
abc transporter
acid
alpha
amino acid
antibody
assembly
bacterial
bacterial proteins
biogenesis
cdna
cytochrome c
cytochrome-c
domain
encode
follow
fraction
function
gene
hydrophobicity
land
land plants
maturation
membrane
membrane protein
mitochondria
mitochondrial
mitochondrial dna
mitochondrial gene
nucleotide
nucleotide sequence
organelle
organism
pathway
plant mitochondria
position
products
protein
rate
region
sequencing
similarities
subunit
transcript
transporter
triticum
triticum aestivum
wheat mitochondria
yeast

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