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GrainGenes Reference Report: BBA-1517-270

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Reference	BBA-1517-270
Title	Cloning of new members of heat shock protein HSP101 gene family in wheat (Triticum aestivum (L.) Moench) inducible by heat, dehydration, and ABA
Journal	Biochimica et Biophysica Acta-Gene Structure and Expression
Year	2001
Volume	1517
Pages	270-277
Author	Campbell JL [ Show all 6 ]
Abstract	Summary: We have cloned two cDNAs, TaHSP101B and TaHSP101C, encoding two heat stress-inducible members of HSP101/ClpB family in bread wheat (Triticum aestivum (L.) Moench.). Proteins encoded by these cDNAs are highly similar at the primary sequence level and diverged from the previously reported TaHSP101 (designated TaHSP101A) both in the consensus ATP/GTP-binding region II and in the carboxy terminal region. The HSP101 gene was determined to be a single copy gene or a member of a small gene family in hexaploid wheat. Messages encoding HSP101 proteins were inducible by heat stress treatments in both wheat leaves and roots. Accumulation of the TaHSP101C mRNA was less abundant than that of TaHSP101B mRNA. We are showing for the first time that in addition to heat stress, expression of HSP101 mRNAs in wheat leaves was induced by a 2-h dehydration and a treatment with 5 x 10(-5)M ABA, but not affected by chilling or wounding, indicating that HSP101 proteins may be involved in both heat and drought responses in wheat
Keyword	[ Hide all but 1 of 35 ] (triticum aestivum) aba bread bread wheat cdna chilling cloning complements dehydration drought drought stress family gene gene family genetic heat heat shock heat shock protein heat stress hexaploid hexaploid wheat maize member mrna root sequence shock-protein single stress terminal region thermotolerance triticum aestivum wheat leaves wounding yeast

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