Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: PDS-84-483

[Submit comment/correction]

Reference	PDS-84-483
Title	Molecular and biological characterization of a trackable Illinois isolate of Barley yellow dwarf virus-PAV
Journal	Plant Disease
Year	2000
Volume	84
Pages	483-486
Author	Moon JS Allen RG Domier LL Hewings AD
Abstract	Summary: In consecutive annual statewide surveys of the incidence of Barley yellow dwarf viruses (BYDVs) in Illinois wheat and oat fields, 27 BYDV-PAV-like isolates were identified. Using polymerase chain reaction (PCR), the coat protein regions of all 27 isolates were analyzed for restriction fragment length polymorphisms. The PCR products of two isolates, one from each year, had restriction fragment profiles after digestion with HaeIII that differed from the other isolates. The nucleotide sequences of the coat protein regions of a laboratory isolate, BYDV-PAV-IL (PAV-IL), two of the isolates with the common restriction profile, and the two isolates with polymorphic profiles were more than 98% identical. The relatively rare isolate identified during the first year was designated BYDV-PAV-DK1 (PAV-DK1) and further characterized biologically. PAV-DK1 and PAV-IL did not differ significantly in symptom expression, but did differ significantly in rates of transmission by two of the three biotypes of Rhopalosiphum padi examined. Since PAV-DK1 does not occur in high levels in the state of Illinois, and its PCR products have a unique restriction enzyme profile, it has the potential to be used as a traceable isolate in field epidemiological experiments
Keyword	[ Hide all but 1 of 38 ] aphid transmission avena sativa barley barley yellow dwarf luteovirus biotypes bydv coat protein digestion disease transmission disease vectors enzyme epidemiology field fragment length polymorphism illinois its luteoviruses nucleotide nucleotide sequence nucleotide-sequences oat pcr pcr-rflp plant height polymerase polymerase chain reaction polymorphism rare readthrough protein rflp rhopalosiphum padi sequence serotype state strain differences surveys symptoms transmission

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.