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GrainGenes Reference Report: PCT-73-177

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Reference	PCT-73-177
Title	Two pathways of plant regeneration in wheat anther culture
Journal	Plant Cell Tissue and Organ Culture
Year	2003
Volume	73
Pages	177-187
Author	Konieczny R Czaplicki AZ Golczyk H Przywara L
Abstract	Summary: The anthers of 10 Polish winter wheat (Triticum aestivum L.) cultivars were used for the induction of androgenesis and plant regeneration. The highest rate of callus induction (9.1%) and green plant production (0.8%) was obtained with the cultivar Apollo that was chosen for histological analysis. The first androgenic division was symmetrical and occurred after 3 weeks of culture. Further divisions of newly formed cells gave rise to multicellular structures which followed two developmental pathways: callus production or direct embryo formation. Plant regeneration was observed in both pathways. Chromosome counting of plantlets regenerated showed that haploid metaphases 2n=3x=21 were the most frequent
Keyword	[ Hide all but 1 of 23 ] androgenesis anther callus callus formation callus induction cell chromosome culture differentiation division embryo genotype gramineae induction metaphase microspore culture organogenesis pollen embryogenesis regeneration somatic embryogenesis triticum winter zygotic embryos

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