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GrainGenes Reference Report: PCT-42-163

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Reference	PCT-42-163
Title	Effects of culture conditions on isolated microspore response of barley cultivar Igri
Journal	Plant Cell, Tissue and Organ Culture
Year	1995
Volume	42
Pages	163-169
Author	Cistue L Ziauddin A Simion E Kasha K
Abstract	Pretreatment of anthers in mannitol prior to isolation of microspores by glass rod homogenization was effective for in vitro induction of embryogenesis in barley cv Igri A procedure for separation of viable microspores using centrifugation on 20% maltose was developed The concentration of microspores was important and greatly increased the number of developing structures Initial culture of microspores on FHG medium containing 62 g l-1 maltose, 4.4 micromolar (1 mg l-1) BA and 200 g l-1 Ficoll-400 resulted in high frequencies of plant regeneration. Albino plant frequency was correlated to length of time in culture. Stock plant condition appeared to be a major factor influencing induction frequency. From 668 to 1738 green plants per 100 anthers were produced. The number of calli and embryos obtained and the number of green plantlets regenerated were improved by increasing the Ficoll concentration from 100 g l-1 to 400 g l-1 during the culture period compared to continuous culture on FHG Ficoll 200 g l-1.
Keyword	[ Hide all but 1 of 12 ] aeration anther-culture callus culture-media hordeum vulgare mannitol osmotic-pressure plant embryos pollen pretreatment regenerative-ability viability

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