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GrainGenes Reference Report: PCP-43-885

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Reference	PCP-43-885
Title	Functional analysis of water channels in barley roots
Journal	Plant and Cell Physiology
Year	2002
Volume	43
Pages	885-893
Author	Katsuhara M Akiyama Y Koshio K Shibasaka M Kasamo K
Abstract	Summary: We identified three genes homologous to water channels in the plasma membrane type subfamily from roots of barley seedlings. These genes were designated HvPIP2;1, HvPIP1;3, and HvPIP1;5 after comparison to Arabidopsis aquaporins. Competitive reverse transcription (RT)-PCR was applied in order to distinguish and to quantify their transcripts. The HvPIP2;1 transcript was the most abundant among the three in roots. Salt stress (200 mM NaCl) down-regulated HvPIP2;1 (transcript and protein), but had almost no effect on the expressions of HvPIP1;3, or HvPIP1;5. Approximately equal amounts of the transcripts of the three were detected in shoots, and salt stress enhanced the expression of HvPIP2;1 but not of HvPIP1;3, or HvPIP1;5. HvPIP2;1 protein was confirmed to be localized in the plasma membrane. Functional expression of HvPIP2;1 in Xenopus oocytes confirmed that HvPIP2;1 encoded an aquaporin that transports water. This water permeability was reduced by HgCl2, which is a typical water channel inhibitor. This activity was not modified by some inhibitors against protein kinase and protein phosphatase
External Databases	Pubmed: 12198191
Keyword	[ Hide all but 1 of 42 ] aquaporin arabidopsis barley barley roots barley seedlings competitive rt-pcr distinguish family functional expression functional-analysis gene genes encoding aquaporins intrinsic proteins kinase membrane nacl oocyte oocytes order permeability phosphatase plasma protein protein kinase protein phosphatase reverse transcription salt seedling stress stresses subfamilies tip transcript transcription transport water water channel water permeability xenopus xenopus oocyte xenopus oocytes xenopus-oocytes

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