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GrainGenes Reference Report: PCP-43-307

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Reference	PCP-43-307
Title	Temporal and spatial expression pattern of the OSVP1 and OSEM genes during seed development in rice
Journal	Plant and Cell Physiology
Year	2002
Volume	43
Pages	307-313
Author	Miyoshi K Kagaya Y Ogawa Y Nagato Y Hattori T
Abstract	Summary: The spatial and temporal expression patterns of the rice VP1 (OSVP1) gene, as well as the OSEM gene which it controls, were studied during seed development by in situ hybridization and immuno-localization techniques. The expression of OSVP1 could be detected in embryos as early as 2-3 d after pollination (DAP) and thereafter became preferentially localized to shoot, radicle and vascular tissues during the embryo development at both the mRNA and protein levels. In the aleurone layers, OSVP1 mRNA and protein were detected after 6 DAP. OSEM mRNA was detectable after 6 DAP in the embryo and aleurone tissue. The spatial distribution within the embryo of OSEM mRNA and OSVP1 mRNA/protein was very similar after 6 DAP. Transgenic rice carrying a beta-glucuronidase (GUS) gene transcribed from a chimeric promoter consisting of the CaMV 35S minimal promoter (-46) and the 55-bp promoter fragment of OSEM, minimally required for ABA and VP1 regulation, also exhibited a spatial pattern of GUS expression similar to that of OSEM and OSVP1. These results suggest that (OS)VP1 is a major determinant not only of the seed specificity but also of the spatial pattern of OSEM expression in the developing seed
Keyword	[ Hide all but 1 of 41 ] aba aleurone aleurone layer beta-glucuronidase chimeric control developing seed developmental stages enzyme activity expression patterns gene gene expression genetic regulation hybridization immunolocalization in situ in situ hybridization layer localization oryza sativa plant anatomy plant embryos pollination promoter protein protein content radicles regulation rice seed seed development shoot spatial distribution spatial pattern specificity tissue transgenic transgenic plant transgenic rice vascular system vascular tissue

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