Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: PCP-43-224

[Submit comment/correction]

Reference	PCP-43-224
Title	gamma-Tubulin in barley and tobacco: Sequence relationship and RNA expression patterns in developing leaves during mitosis and post-mitotic growth
Journal	Plant and Cell Physiology
Year	2002
Volume	43
Pages	224-229
Author	Schroder J Kautz K Wernicke W
Abstract	Summary: gamma-Tubulin is typically associated with microtubule organising centres, such as the centrosome, and appears to mediate microtubule nucleation. Centrosomes are usually not found in higher plants, but active genes homologous to gamma-tubulin have been identified in the plant kingdom, including the angiosperms Arabidopsis, maize and rice. We have isolated and characterised gamma-tubulin cDNA sequences of two further angiosperm species, barley and tobacco. Sequence comparison revealed a phylogenetic tree with distinct clusters corresponding to the systematic position of the species. Furthermore, domains, thought to be exposed in the folded protein and to be candidates for interaction with associated, nucleation-site related proteins, exhibited motifs highly specific of multicellular plants. Strong expression of the gamma-tubulin genes, as determined by Northern blotting, correlated with mitotic activity. Expression dropped distinctly when mitotic activity ceased. Thus, in post-mitotic tissues that showed intricate reshuffling of cortical microtubule arrays related to cell shaping only very low gamma-tubulin steady-state RNA levels were found, contrasting with the situation for alpha-tubulin. The findings indicate that gamma-tubulin expression in plants may be more tightly linked to mitosis, although there is some gamma-tubulin expression at the RNA level even after mitosis. It follows that the post-mitotic changes in microtubular arrays may be less dependent on concurrent gamma-tubulin RNA expression than mitotic cells
External Databases	Pubmed: 11867702
Keyword	[ Hide all but 1 of 49 ] alpha-tubulin angiosperm angiosperms arabidopsis arrays barley cdna cdna sequence cell cell shaping centrosome cluster cortical microtubule cytoskeleton domain expression patterns follow gamma-tubulin gene gene expression growth higher plant hordeum vulgare maize microtubular arrays microtubule arrays microtubule nucleation microtubule organising centres mitosis mitotic-activity motif motifs nicotiana tabacum northern northern blotting nucleation pattern phylogenetic tree phylogeny plant kingdom position protein rice sequence systematic position tissue to-cell tobacco tree

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.