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GrainGenes Reference Report: PCP-42-1088

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Reference	PCP-42-1088
Title	An isozyme of betaine aldehyde dehydrogenase in barley
Journal	Plant and Cell Physiology
Year	2001
Volume	42
Pages	1088-1092
Author	Nakamura T [ Show all 6 ]
Abstract	Summary: Betaine aldehyde dehydrogenase (BADH) is an important enzyme for Gly betaine synthesis. We isolated two types of BADH cDNAs (BBD1 and BBD2) from barley. As BBD1 contained the signal sequence (SKL) targeting to microbodies, BBD2 was more similar to previously reported genes coding for BADH in dicotyledons (chloroplast type) than those in monocotyledons (microbody type). The two barley BADH genes showed different expression patterns. The BBD1 transcript was more abundant in roots than leaves and was induced to higher levels by salt, drought and abscisic acid (ABA) treatment. BBD2 transcript was more abundant in leaves and induced by salt, drought, PEG and ABA treatment. To understand the processing of these BADH proteins, we partially purified both enzymes and determined their N-terminal sequences. Based on comparisons of the N-terminal sequences to their deduced amino acid sequence, neither BBD1 nor BBD2 is processed at the N-terminus. These results suggest that BBD2 codes for a new type of BADH, which is not localized in either chloroplasts or mitochondria
External Databases	Pubmed: 11673624
Keyword	[ Hide all but 1 of 46 ] aba abscisic acid acid aldehyde aldehyde dehydrogenase amino acid amino acid sequence barley betaine betaine aldehyde betaine aldehyde dehydrogenase cdna cdna cloning chloroplast choline monooxygenase dehydrogenase drought efficiently catalyzes enzyme expression patterns gene gly betaine glycinebetaine isozyme microbodies mitochondria molecular cloning monocotyledon monocotyledons n-terminal sequence n-terminus nor osmotic-stress pattern processing protein root salinity stress salt sequence signal spinach leaves sugar beet synthesis targeting transcript

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