GrainGenes Reference Report: PBR-123-428
Reference
PBR-123-428
Title
RAPD markers linked with restorer genes for the C-source of cytoplasmic male sterility in rye (Secale cereale L.)
Journal
Plant Breeding
Year
2004
Volume
123
Pages
428-433
Author
Stojalowski S Lapinski M Masojc P
Abstract
The study was aimed at the identification of random amplified polymorphic DNA markers linked to genes controlling male sterility in rye with the C-source of sterility-inducing cytoplasm. Markers of male sterility were distinguished using bulk segregant analysis, carried out on the two F SUB 2 crosses between male sterile and male fertile inbred lines. Screening of polymorphisms revealed by 1000 arbitrary 10-mer primers allowed the detection of 10 markers in the cross between 711-cmsC and DS2 lines and seven markers in the cross between 544-cmsC and Ot0-20 lines. Five markers were common for the two crosses, which allowed comparative mapping to be performed. Ten markers were mapped on the 4RL chromosome arm where two linked quantitative trait loci (QTL) for male sterility were discovered. Additional QTL of minor effect on male fertility were detected between the two linked markers provisionally assigned to the 6RS chromosome arm. The effectiveness of the marker-assisted selection (MAS) for male-sterile genotypes was evaluated
Keyword
[ Hide all but 1 of 6 ] cytoplasmic male sterility genes male sterility restorer genes rye sterility
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: PBR-123-428
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||