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GrainGenes Reference Report: PBR-123-220

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Reference	PBR-123-220
Title	The study of introgressive lines of Triticum aestivum x Aegilops speltoides by in situ and SSR analyses
Journal	Plant Breeding
Year	2004
Volume	123
Pages	220-224
Author	Adonina IG Salina EA Efremova TT Pshenichnikova TA
Abstract	Summary: Fluorescence in situ hybridization (FISH) with a genome-specific repeat, Spelt1, and wheat simple sequence repeat (SSR) markers were used to analyse the chromosome constitution of two Triticum aestivum x Aegilops speltoides introgressive lines. The lines 170/98i and 178/98i carried one and two subtelomeric regions of Ae. speltoides (per haploid genome), respectively, marked by Spelt1 repeats according to FISH data. SSR analysis detected homoeologous substitution of wheat chromosome 7D with Ae. speltoides chromosome 7S in the lines 178/98i and 170/98i as well as the assumed terminal translocation in the short arm of chromosome 3A in the line 178/98i. Anthocyanin pigmentation of the coleoptiles was found in the lines 170/98i and 178/98i and resulted from the 7S (7D) substitution. It was demonstrated that Spelt1 could be effectively used for the rapid identification (without DNA isolation) of terminal translocations of T. aestivum x Ae. speltoides introgressive lines as well as for further analysis of the stability of the hybrid plants
Keyword	[ Hide all but 1 of 30 ] addition line aegilops aegilops speltoides anthocyanin pigmentation barley dna dna isolation fish fluorescence fluorescence in situ hybridization genome haploid hexaploid bread wheat hybrid hybridization in situ in situ hybridization introgressive lines microsatellite pigmentation sequence simple sequence repeat spelt1 subtelomeric repeat speltoides ssr ssr analysis stability substitution substitution line triticum

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