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GrainGenes Reference Report: PBR-123-198

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Reference	PBR-123-198
Title	Identification of resistance gene analogue markers closely linked to wheat powdery mildew resistance gene Pm31
Journal	Plant Breeding
Year	2004
Volume	123
Pages	198-200
Author	Xie C [ Show all 6 ]
Abstract	Summary: Specific oligonucleotide primers, designed for the sequences of known plant disease resistance genes, were used to amplify resistance gene analogues (RGAs) from wheat genomic DNA. This method was applied in a bulked segregant analysis to screen for the RGA markers linked to the powdery mildew resistance gene Pm31, introgressed into common wheat from wild emmer. Two RGA markers (RGA200 and RGA390) were found to be closely linked to Pm31 and completely co-segregating with the marker allele of Xpsp3029 linked to Pm31, with a genetic distance of 0.6 cM. These two RGA markers were then integrated into the formerly established microsatellite map of Pm31 region. The result showed the effectiveness of the RGA approach for developing molecular markers linked to disease resistance genes and demonstrated the efficiency of denaturing polyacrylamide-gel electrophoresis for detecting polymerase chain reaction polymorphism
Keyword	[ Hide all but 1 of 41 ] allele apr barley bulked segregant analysis chain chain-reaction chromosomal location cm common wheat disease disease resistance disease resistance gene distance dna electrophoresis erysiphe graminis gene genomic dna locus map microsatellite mildew plant disease plant disease resistance polyacrylamide gel electrophoresis polymerase polymorphism powdery mildew powdery mildew resistance primer resistance resistance gene resistance gene analogue rga rice sequence specific triticum aestivum wheat powdery mildew wild wild emmer

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