GrainGenes Reference Report: PBR-123-198
Reference
PBR-123-198
Title
Identification of resistance gene analogue markers closely linked to wheat powdery mildew resistance gene Pm31
Journal
Plant Breeding
Year
2004
Volume
123
Pages
198-200
Author
[ Hide all but 1 of 6 ] Xie C Sun Q Ni Z Yang T Nevo E Fahima T
Abstract
Summary: Specific oligonucleotide primers, designed for the sequences of known plant disease resistance genes, were used to amplify resistance gene analogues (RGAs) from wheat genomic DNA. This method was applied in a bulked segregant analysis to screen for the RGA markers linked to the powdery mildew resistance gene Pm31, introgressed into common wheat from wild emmer. Two RGA markers (RGA200 and RGA390) were found to be closely linked to Pm31 and completely co-segregating with the marker allele of Xpsp3029 linked to Pm31, with a genetic distance of 0.6 cM. These two RGA markers were then integrated into the formerly established microsatellite map of Pm31 region. The result showed the effectiveness of the RGA approach for developing molecular markers linked to disease resistance genes and demonstrated the efficiency of denaturing polyacrylamide-gel electrophoresis for detecting polymerase chain reaction polymorphism
Keyword
allele
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: PBR-123-198
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||