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GrainGenes Reference Report: PBR-122-204

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Reference	PBR-122-204
Title	Identification of a molecular marker linked to an Agropyron elongatum-derived gene Lr19 for leaf rust resistance in wheat
Journal	Plant Breeding
Year	2003
Volume	122
Pages	204-208
Author	Cherukuri DP [ Show all 8 ]
Abstract	Summary: The leaf rust resistance gene Lr19, transferred from Agropyron elongatum into wheat (Triticum aestivum L.) imparts resistance to all pathotypes of leaf rust (Puccinia recondita f.sp. tritici) in South-east Asia. A segregating F2 population from a cross between the leaf rust resistant parent 'HW 2046' carrying Lr19 and a susceptible parent 'Agra Local' was screened in the phytotron against a virulent pathotype 77-5 of leaf rust with the objective of identifying the molecular markers linked to Lr19. The gene was first tagged with a randomly amplified polymorphic DNA (RAPD) marker S73(728). The RAPD marker linked to the gene Lr19 which mapped at 6.4 +/- 0.035 cM distance, was converted to a sequence characterized amplified region (SCAR) marker. The SCAR marker (SCS73(719)) was specific to Lr19 and was not amplified in the near-isogenic lines (NILs) carrying other equally effective alien genes Lr9, Lr28 and Lr32 enabling breeders to pyramid Lr19 with these genes
Keyword	[ Hide all but 1 of 38 ] agropyron agropyron elongatum amplified polymorphic dna arbitrary primers asia bulked segregant analysis conferring resistance disease resistance dna elongatum f-2 population gene leaf rust leaf rust resistance lr19 lr24 marker marker-assisted selection near isogenic lines nil pcr population puccinia recondita puccinia recondita f .sp tritici rapd rapd marker resistance resistance gene resistant rust rust resistance rust-resistance scar marker sequence south east asia tritici triticum triticum aestivum

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