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GrainGenes Reference Report: PBR-122-83

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Reference	PBR-122-83
Title	Amplified fragment length polymorphism-derived microsatellite sequence linked to the Pch1 and Ep-D1 loci in common wheat
Journal	Plant Breeding
Year	2003
Volume	122
Pages	83-85
Author	Groenewald JZ Marais AS Marais GF
Abstract	Summary: Amplified fragment length polymorphism (AFLP) markers linked to the Aegilops ventricosa-derived chromosome segment in 'VPM1' on which the eyespot resistance gene, Pch1, and the endopeptidase gene, Ep-D1b, occur were identified. One marker was isolated from the gel, cloned and sequenced. Sequence analysis revealed a microsatellite repeat motif. Sequence-specific primers were designed to amplify a product containing the repeat motif, and the microsatellite marker was tested for cosegregation with the Ep-D1b allele. Distinct alleles were produced by the Pch1 sources, normal wheat and wheat containing the Lr19 translocation. A recombination frequency of 0.02 was calculated between the microsatellite marker and Ep-D1
Keyword	[ Hide all but 1 of 30 ] aegilops aflp allele amplified fragment length polymorphism barley chromosome chromosome-7d common wheat endopeptidase eyespot pseudocercosporella-herpotrichoides eyespot resistance fragment length polymorphism gel gene genetic mapping length polymorphism locus map microsatellite microsatellite marker polymorphism primer recombination repeat resistance resistance gene sequence source translocation triticum aestivum

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