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GrainGenes Reference Report: PBR-122-52

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Reference	PBR-122-52
Title	Marker-assisted backcross introgression of the Yd2 gene conferring resistance to barley yellow dwarf virus in barley
Journal	Plant Breeding
Year	2003
Volume	122
Pages	52-56
Author	Jefferies SP [ Show all 6 ]
Abstract	Summary: YLM, a codominant polymerase chain reaction (PCR) marker linked to Yd2, could substantially improve the precision and efficiency of barley yellow dwarf virus (BYDV) resistance breeding. The aim of this study was to assess the effectiveness of YLM in a marker-assisted introgression programme and to quantify associations between the presence of Yd2 and other agronomic and quality traits. The Yd2 gene was introgressed into a BYDV-susceptible background through two cycles of marker-assisted backcrossing. BC2 F2-derived lines, either carrying or not carrying the YLM allele associated with resistance, were compared in the presence and absence of BYDV. The YLM marker was shown to be effective in the introgression of Yd2. Lines carrying the YLM allele associated with resistance produced significantly fewer leaf symptoms and showed a reduction in yield loss when infected with BYDV. There were no deleterious effects associated with the introgression of Yd2 on grain yield, grain size or malting quality. The implications of marker-assisted selection for Yd2 on barley improvement are discussed
Keyword	[ Hide all but 1 of 31 ] associations backcross backcrossing barley barley leaf scald barley yellow dwarf barley yellow dwarf virus breeding bydv conferring resistance cycle dwarf gene hordeum vulgare improvement introgression malting quality marker pcr polymerase quality quality traits reduction resistance selection symptoms virus yd2 yellow dwarf virus yield yield loss

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