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GrainGenes Reference Report: PBR-121-325

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Reference	PBR-121-325
Title	Identification of a microsatellite marker associated with Pm3 resistance alleles to powdery mildew in wheat
Journal	Plant Breeding
Year	2002
Volume	121
Pages	325-329
Author	Bougot Y Lemoine J Pavoine MT Barloy D Doussinault G
Abstract	Summary: The Pm3 resistance locus, located on chromosome 1A in wheat, confers race-specific resistance to the obligate biotrophic fungus Blumeria graminis (DC) E.O. Speer f. sp. tritici, the causal agent of powdery mildew. Several Pm3 alleles are still effective in controlling the disease in Europe. A genetic map was constructed to map the Pm3g allele in the recombinant inbred line progeny from the cross 'RE9001' (susceptible) x 'Courtot' (resistant). Two microsatellite markers were closely mapped to Pm3g. The PSP2999 marker, which cosegregates with this allele, was shown to detect the presence of the Pm3g resistance allele in other cultivars. A collection of 56 wheat cultivars or advanced lines carrying one Pm3 allele was used to assess the allele-specific amplification of the PSP2999 marker. The same amplification pattern was obtained for lines with Pm3a, Pm3b, Pm3e, Pm3f and Pm3g alleles. Twenty genotypes carrying Pm3d showed a specific amplification pattern. This marker allowed the detection of the Pm3d allele in highly resistant lines whose resistance gene combinations were unknown. It was concluded that PSP2999 is a useful marker to detect Pm3 alleles in parents and to manage them in breeding programmes
Gene	Pm3 (Triticum)
Locus	Pm3
Keyword	[ Hide all but 1 of 44 ] allele allele-specific amplification amplification blumeria blumeria graminis bread wheat breeding breeding programme causal causal agent chromosomal location chromosome chromosome-1a collection combinations common wheat detect detection disease europe f-sp tritici fungus gene gene mapping graminis inbred line locus map microsatellite mildew molecular markers pattern powdery mildew progeny race-specific resistance recombinant recombinant inbred line resistance resistance gene resistant specific amplification tritici triticum aestivum wheat cultivar

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