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GrainGenes Reference Report: PBR-121-218

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Reference	PBR-121-218
Title	Amplified fragment length polymorphism- and simple sequence repeat-based molecular tagging and mapping of greenbug resistance gene Gb3 in wheat
Journal	Plant Breeding
Year	2002
Pages	218-223
Author	Weng Y Lazar MD
Abstract	Summary: The greenbug, Schizaphis graminum (Rondani), is the most economically damaging aphid pest of wheat in the southern Great Plains of the USA. In this study, the single, dominant greenbug resistance gene, Gb3, was molecularly tagged and genetically mapped using amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers. Three AFLP loci were associated with the Gb3 locus in linkage analysis with 75 F(2:3) families from the cross between two near-isogenic lines (NILs) for Gb3, 'TXGBE273' and 'TXGBE281'. Two of these loci, XMgccPagg and XMaggPatg cosegregate with Gb3 in the population analysed. Further analysis indicated that XMgccPagg and XMaggPatg are specific for the Gb3 locus in diverse genetic backgrounds. Two SSR markers, Xgwm111 and Xgwm428 previously mapped in wheat chromosome 7D, were shown to be linked with Gb3, 22.5 cM and 33.1 cM from Gb3, respectively, in an F2 population of 'Largo' x 'TAM 107', suggesting that Gb3 is located in the long arm of chromosome 7D. The two AFLP markers cosegregating with Gb3 are valuable tools in developing molecular markers for marker-assisted selection of greenbug resistance in wheat breeding
Keyword	[ Hide all but 1 of 41 ] aflp aflp marker amplified fragment length polymorphism aphid breeding chromosome chromosome maps chromosome-7d family fragment length polymorphism gene genetic mapping genetic marker great-plains inheritance length polymorphism linkage linkage analysis linkage groups mapping microsatellite molecular tagging near-isogenic line nil pest pest resistance polymorphism population repeat resistance resistance gene schizaphis graminum selection sequence simple sequence repeat ssr ssr marker tool tools triticum aestivum wheat breeding

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