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GrainGenes Reference Report: PBR-121-17

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Reference	PBR-121-17
Title	Identification of microsatellite polymorphisms in an expressed portion of the rye genome.
Journal	Plant Breeding
Year	2002
Volume	121
Pages	17-25
Author	Hackauf B Wehling P
Abstract	For genetic and applied purposes the number of SSR markers in rye has to be significantly increased to cover the entire genome. To this end, more than 8000 publicly accessible rye cDNA sequences from anthers, cold-stressed leaves, and aluminum-stressed as well as unstressed roots were exploited as a resource for SSR marker development. One hundred and fifty-seven Secale cereale microsatellite (SCM) loci out of 528 simple sequence repeats comprising di-, tri- and tetra-nucleotide motifs could be assayed on automated sequencers. One hundred EST-derived SCM loci displayed a length polymorphism among a sample of 15 rye accessions. Forty-five percent of the SCM could be associated with proteins of known or unknown function. As a conclusion, recently published ESTs from different rye tissues proved to be a valuable resource for SSR marker development in rye.
Probe	[ Hide all but 1 of 157 ] SCM1 SCM3 SCM4 SCM6 SCM7 SCM8 SCM10 SCM11 SCM12 SCM13 SCM14 SCM15 SCM16 SCM17 SCM18 SCM19 SCM20 SCM21 SCM22 SCM23 SCM24 SCM26 SCM27 SCM29 SCM30 SCM31 SCM32 SCM33 SCM34 SCM35 SCM36 SCM37 SCM38 SCM41 SCM42 SCM44 SCM45 SCM46 SCM47 SCM48 SCM49 SCM50 SCM51 SCM54 SCM55 SCM56 SCM57 SCM60 SCM61 SCM62 SCM63 SCM64 SCM66 SCM67 SCM68 SCM70 SCM71 SCM73 SCM76 SCM77 SCM78 SCM79 SCM80 SCM81 SCM82 SCM83 SCM84 SCM85 SCM87 SCM88 SCM89 SCM90 SCM91 SCM92 SCM93 SCM94 SCM95 SCM98 SCM99 SCM100 SCM103 SCM106 SCM107 SCM108 SCM110 SCM112 SCM113 SCM114 SCM115 SCM116 SCM117 SCM118 SCM119 SCM121 SCM122 SCM123 SCM124 SCM125 SCM126 SCM127 SCM129 SCM130 SCM131 SCM132 SCM133 SCM134 SCM135 SCM136 SCM137 SCM139 SCM140 SCM141 SCM142 SCM143 SCM144 SCM145 SCM146 SCM147 SCM148 SCM149 SCM150 SCM151 SCM152 SCM153 SCM154 SCM155 SCM156 SCM157 SCM158 SCM159 SCM160 SCM161 SCM162 SCM163 SCM164 SCM165 SCM166 SCM167 SCM168 SCM169 SCM170 SCM171 SCM172 SCM173 SCM174 SCM175 SCM176 SCM177 SCM178 SCM179 SCM181 SCM182 SCM183 SCM184 SCM185 SCM186 SCM188
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