GrainGenes Reference Report: PBR-120-497
Reference
PBR-120-497
Title
Expressed sequence tags from cold-acclimatized barley can identify novel plant genes
Journal
Plant Breeding
Year
2001
Pages
497-502
Author
Faccioli P Pecchioni N Cattivelli L Stanca AM Terzi V
Abstract
Summary: Expressed sequence tags (ESTs) databases are being created for several plant species. ESTs have the potential to analyse the expressed part of the genome, and to be used in a wide range of applications from the theoretical aspect of plant biology to the breeding process. The present study is based on a cold-stressed cDNA library as a source of ESTs in barley: partial cDNA sequencing of randomly isolated clones yielded 960 ESTs. The strategy of random sequencing cDNA clones at their 5' ends allows one to obtain information on the steady-state level of the transcripts in a cold-stressed green leaf of barley. Repetitive sequencing of nearly identical ESTs provides, in addition, useful information on the existence of multigene families. The results of a BLAST search performed on this set of ESTs showed that 18.5% of the tags exhibited no significant homology to known genes, 10% had significant levels of homology with known cold or other stress-related genes, about 26% proved homologous to enzymes involved in several metabolic pathways and about 15% encoded for ribosomal proteins, cytoskeleton proteins, histones, ubiquitin, elongation factors and others. Redundancy was found for some classes of tag: RuBisCo, cab proteins, carbonic anhydrase, glutamate decarboxylase and stress related proteins
Keyword
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: PBR-120-497
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||