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GrainGenes Reference Report: PBR-120-448

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Reference	PBR-120-448
Title	An STS marker distinguishing the rye-derived powdery mildew resistance alleles at the Pm8/Pm17 locus of common wheat
Journal	Plant Breeding
Year	2001
Volume	120
Pages	448-450
Author	Mohler V Hsam SLK Zeller FJ Wenzel G
Abstract	Summary: A sequence-tagged site marker has been developed from restriction fragment length polymorphism marker probe IAG95 for the rye-derived powdery mildew resistance Pm8/Pm17 locus of common wheat. This polymerase chain reaction marker enables the amplification of DNA fragments with different sizes from T1AL.1RS and T1BL.1RS wheat-rye translocation cultivars with chromatin from 'Insave' and 'Petkus' rye, respectively, and therefore will be very useful in distinguishing Pm8-carrying cultivars from Pm17-carrying cultivars. Results obtained with that marker were compared with resistance tests performed on detached primary leaves of 29 wheat lines from two populations derived from doubled haploid production. The molecular assay corresponded well with the resistance tests in all the lines, and therefore will be helpful for the identification of Pm17 in lines in which other Pm genes or quantitative trait loci are present
Keyword	[ Hide all but 1 of 62 ] allele alleles amplification assay chain chromatin common common wheat cultivar disease resistance genes dna dna fragment dna fragments doubled haploid fragment fragment length polymorphism gene haploid haploid production identification leaf length length polymorphism line loci locus marker mildew mildew resistance molecular molecular markers pcr pm17 polymerase polymerase chain reaction polymorphism population powdery mildew powdery mildew resistance primary leaves production quantitative trait quantitative trait loci resistance resistance tests restriction restriction fragment length restriction fragment length polymorphism rye secale cereale sequence-tagged-site site sizes sts sts marker tests trait trait loci translocation triticum aestivum wheat wheat-rye translocation

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