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GrainGenes Reference Report: PBR-120-445

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Reference	PBR-120-445
Title	Segregation for isozymes and HMW glutenins in a doubled-haploid cross of winter wheat carrying 1BL.1RS and 5DL.5RS translocations from rye
Journal	Plant Breeding
Year	2001
Volume	120
Pages	445-447
Author	Vahl U Muller G Weber WE
Abstract	Summary: The doubled haploid (DH) wheat line 'dh 5841' carrying two translocations from rye, 5DL.5RS and 1BL.1RS, has been crossed to the subline of wheat cultivar 'Amadeus 7143' with a 1BL.1RS translocation. The resulting F1 hybrid IJ 98 with a heterozygous 5DL.5DS-5DL.5RS chromosome pair has been used to produce doubled haploids. A total of 57 DH lines were obtained from plantlets regenerated in anther culture after successful colchicine treatment and seed set. These lines were identified regarding the constitution of chromosome 5D (5DL.5DS or 5DL.5RS) by means of isoenzyme marker analysis. Thirty DH lines possessed the 5DL.5DS chromosome, while the remaining 27 lines carried the 5DL.5RS translocation. For some of these lines, the 5DL.5RS chromosome was cytologically confirmed by C-banding. Furthermore, the DH lines were evaluated for their high molecular weight glutenin subunit composition. All possible combinations for the four independent loci - Skdh, Glu-A1, Glu-B1 and Glu-D1 - were detected in only 57 DH lines and no segregation distortion was observed
Keyword	[ Hide all but 1 of 56 ] 1bl.1rs ability analysis anther anther culture c-banding chromosome chromosome-translocation colchicine colchicine treatment composition cross cultivar culture dh lines distortion doubled haploid doubled haploid lines glu-a1 glu-d1 glutenin glutenin subunit glutenin subunit composition haploid high molecular weight high molecular weight glutenin subunit hmw hmw glutenin hmw glutenin subunits hybrid identification isoenzyme isozyme line loci marker molecular molecular weight powdery mildew resistance rye secale cereale seed seed set segregation segregation distortion subunit subunit composition translocation triticum aestivum weight weight glutenin subunit wheat wheat cultivar wheat-rye translocation winter winter wheat

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