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GrainGenes Reference Report: PBR-120-301

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Reference	PBR-120-301
Title	Mapping of a gene for leaf scald resistance in barley line 'B87/14' and validation of microsatellite and RFLP markers for marker-assisted selection
Journal	Plant Breeding
Year	2001
Volume	120
Pages	301-304
Author	Williams K Bogacki P Scott L Karakousis A Wallwork H
Abstract	Summary: Seedlings of the barley line 'B87/14' were resistant to 22 out of 23 Australian isolates of Rhynchosporium secalis, the causal agent of leaf scald. 'B87/14'-based populations were developed to determine the location of the resistance locus. Scald resistance segregated as a single dominant trait in BC1F2 and BC1F3 populations. Bulked segregant analysis identified amplified fragment length polymorphisms (AFLPs) with close linkage to the resistance locus. Fully mapped populations not segregating for scald resistance located these AFLP markers on chromosome 3H, possibly within the complex Rrs1 scald locus. Microsatellite and restriction fragment length polymorphism markers adjacent to the AFLP markers were identified and validated for their linkage to scald resistance in a second segregating population, with the closest marker 2.2 cM from the resistance locus. These markers can be used for selection of the Rrs.B87 scald-resistance locus, and other genes at the chromosome 3H Rrs1 locus
Locus	BCD828
Keyword	[ Hide all but 1 of 42 ] aflp aflp marker aflps amplified fragment length polymorphism barley bulked segregant analysis causal agent chromosome complexes dominant fragment length polymorphism gene hordeum vulgare isolate isozyme leaf scald length polymorphism linkage locus map mapping marker marker-assisted selection microsatellite molecular markers polymorphism population resistance resistant restriction restriction fragment length rflp rflp marker rhynchosporium rhynchosporium secalis scald scald resistance seedling segregant analysis selection sequence trait

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