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GrainGenes Reference Report: PBR-120-285

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Reference	PBR-120-285
Title	Effect of the ph1b mutant on chromosome pairing in hybrids between Dasypyrum villosum and Triticum aestivum
Journal	Plant Breeding
Year	2001
Volume	120
Pages	285-289
Author	Yu MQ Deng GB Zhang XP Ma XR Chen J
Abstract	Summary: Chromosome pairing was analysed in F1 hybrids of the wheat cultivar 'Chinese Spring' (CS) and its ph1b mutant (CS ph1b) with Dasypyrum villosum. On average, 1.61 chromosomes per cell paired in the hybrid CS x D. villosum, but 14.43 in the hybrid CS ph1b x D. villosum. Genomic fluorescence in situ hybridization (GISH) revealed three types of homoeologous association between wheat (W) and D. villosum (D) chromosomes (W-D, D-W-W and D-W-D) in pollen mother cells of the CS ph1b x D. villosum hybrid, and only one type (W-W), in the CS x D. villosum hybrid. Both F1 hybrids were self-sterile. The seed set of the backcross of CS x D. villosum with CS was 6.67% and that of CS ph1b x D. villosum with CS or CS ph1b was only 0.45%. The chromosome number of BC1 plants varied from 48 to 72. Translocations of chromosome segments or entire arms between wheat and D. villosum chromosomes were detected by GISH in the BC1 plants from the backcross of CS ph1b x D. villosum to CS ph1b
Keyword	[ Hide all but 1 of 32 ] arms backcross cell chinese chinese spring chromosome chromosome number chromosome pairing chromosome segments cultivar dasypyrum villosum fluorescence fluorescence in situ hybridization gish hybrid hybridization in situ in situ hybridization mutant pairing ph1b mutant pollen pollen mother cells seed seed set segment situ hybridization spring translocation triticum triticum aestivum wheat cultivar

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