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GrainGenes Reference Report: PBR-120-227

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Reference	PBR-120-227
Title	Genome-specific markers of tetraploid wheats and their putative diploid progenitor species
Journal	Plant Breeding
Year	2001
Volume	120
Pages	227-232
Author	Khlestkina EK Salina EA
Abstract	Summary: The objective of this study was to isolate genome-specific markers from the genomes of tetraploid wheats and the putative donor diploid species on the basis of random amplified polymorphic DNA analysis followed by cross-hybridization. Twenty different Triticum and Aegilops species and accessions were analysed by polymerase chain reaction (PCR) using 30 random primers. The polymorphic PCR fragments were then isolated, labelled and used in cross-hybridization screenings. The hybridization results established that one marker was specific to the Ae. speltoides S genome, two to the A genome, one to the B genome and five to the G genomes of polyploid species (and to the genomes of the corresponding progenitor species). Four markers were identified that were specific to both the B and G genomes. Analysis of the Triticum and Aegilops species and accessions supported the notion that Ae. speltoides is more closely related to the B and G genomes of polyploid wheat species than were other members of the Sitopsis section. The data also indicated that the B and G genomes had originated from different accessions of Ae. speltoides
Keyword	[ Hide all but 1 of 39 ] a genome accessions aegilops amplified polymorphic dna b genome chain dna dna analysis dna sequences evolution fragments g genome genome genome relations genomes hybridization isolate land marker member pcr phylogeny plant-science polymerase polymerase chain reaction polymorphic polymorphic dna polymorphic dna analysis polyploid polyploid wheats primer progenitor random amplified polymorphic dna rapd repeated nucleotide sequences screening speltoides tetraploid wheat triticum

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