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GrainGenes Reference Report: PBR-118-88

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Reference	PBR-118-88
Title	Use of multiparental inbred populations to determine allelic relationships of molecular markers.
Journal	Plant Breeding
Year	1999
Volume	118
Pages	88-90
Author	Enjalbert J Boeuf C Belcram H Leroy P
Abstract	The assessment of polymorphism exhibited by molecular markers is an arduous but essential task that facilitates the use of molecular tools by breeders and geneticists. For that purpose, the value of a wheat composite population was assessed for characterizing the diversity of restriction fragment length polymorphism (RFLP) markers developed by INRA-Genoble. The polymorphism of 13 genomic probes was measured over a set of 80 inbred lines randomly extracted by single-seed descent from a composite-cross of 16 wheat lines. The 13 probe-enzyme combinations revealed 27 loci with codominant polymorphism. As many bands were so far unmapped, the segregational analysis of the progenies appeared very suitable for complex patterns, both in determining allelic relationships and in revealing linkage between loci. Allelic diversity, band sizes and chromosomal location assessed from nullisomic-tetrasomic lines are given for the 27 loci.
External Databases	Agricola: 2011-89360
Keyword	[ Hide all but 1 of 16 ] aegilops ventricosa alleles codominance dna probes gene location genetic diversity genetic markers inbred lines intergeneric hybridization interspecific hybridization loci molecular mapping rflp segregation triticum aestivum triticum durum

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