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GrainGenes Reference Report: AUM-59-1289

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Reference
AUM-59-1289
Title
Application of RAPD linkage map for identification of sprouting resistance genes in rye ( Secale cereale L.)
Journal
Annales Universitatis Mariae Curie-Skodowska.Sectio E, Agricultura
Year
2004
Volume
59
Pages
1289-1296
Author
Myskow B
Stojaowski S
Milczarski P
Masojc P
Abstract
The aim of this paper was the construction of random amplified polymorphic DNA linkage map based on the intercross S120 x S76 and localization of quantitative trait loci (QTLs) underlying sprouting resistance in rye. A total of 876 10-mer primers were used for the detection of inter-line polymorphism. Approximately 48 markers were found which segregated in the F SUB 2 progeny according to a single gene model. The map constructed using these markers consisted of ten linkage groups spanning the distance of 248.8 cM and covering 20% of rye genome. Among the mapped markers, three were common with those found earlier on the map constructed using DS2 x RXL10 intercross. A low number of common markers for the two maps precluded chromosomal identification of the newly obtained linkage groups. Two QTLs controlling sprouting resistance were identified within two different linkage groups on the S120 x S76 map
Keyword
[ Hide all but 1 of 15 ]
detection
dna
genetic mapping
genetic marker
linkage
linkage groups
localization
locus
map
progeny
quantitative trait loci
random amplified polymorphic dna
resistance
rye
sprouting

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